t7 endonuclease i cleavage assay Search Results


t7 endonuclease 1  (New England Biolabs)
96
New England Biolabs t7 endonuclease 1
Generation of USP11 gene disruption in human induced pluripotent stem cells (hiPSCs). (A) The location of designed sgRNAs targeting USP11 gene. (B) The efficiency of sgRNAs targeting USP11 was validated by western blot using the USP11 antibody. (C) The USP11 knockout clone in hiPSCs cell line was screened by T7 endonuclease 1 <t>(T7E1)</t> assay. The T7E1 positive clones showing cleavage are represented in red color. The arrowhead indicates the cleaved bands of the polymerase chain reaction (PCR) amplicons. (D) Western blot analysis showing USP11 knockout efficiency in hiPSCs cells. (E, F) The hiPSC-wildtype and hiPSC-USP11KO clones were characterized by (E) quantitative real-time reverse transcription-PCR using USP11 gene primers and (F) AP staining, Scale bar=200 μm. Data are presented as the mean and SD of three independent experiments (n=3). Two-way ANOVA followed by Tukey’s post hoc test was used to analyze. ***p<0.001.
T7 Endonuclease 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dnase  (Worthington Biochemical)
99
Worthington Biochemical dnase
Generation of USP11 gene disruption in human induced pluripotent stem cells (hiPSCs). (A) The location of designed sgRNAs targeting USP11 gene. (B) The efficiency of sgRNAs targeting USP11 was validated by western blot using the USP11 antibody. (C) The USP11 knockout clone in hiPSCs cell line was screened by T7 endonuclease 1 <t>(T7E1)</t> assay. The T7E1 positive clones showing cleavage are represented in red color. The arrowhead indicates the cleaved bands of the polymerase chain reaction (PCR) amplicons. (D) Western blot analysis showing USP11 knockout efficiency in hiPSCs cells. (E, F) The hiPSC-wildtype and hiPSC-USP11KO clones were characterized by (E) quantitative real-time reverse transcription-PCR using USP11 gene primers and (F) AP staining, Scale bar=200 μm. Data are presented as the mean and SD of three independent experiments (n=3). Two-way ANOVA followed by Tukey’s post hoc test was used to analyze. ***p<0.001.
Dnase, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dnase (dnase i, 30 u/ μ g total rna)  (Qiagen)
90
Qiagen dnase (dnase i, 30 u/ μ g total rna)
Generation of USP11 gene disruption in human induced pluripotent stem cells (hiPSCs). (A) The location of designed sgRNAs targeting USP11 gene. (B) The efficiency of sgRNAs targeting USP11 was validated by western blot using the USP11 antibody. (C) The USP11 knockout clone in hiPSCs cell line was screened by T7 endonuclease 1 <t>(T7E1)</t> assay. The T7E1 positive clones showing cleavage are represented in red color. The arrowhead indicates the cleaved bands of the polymerase chain reaction (PCR) amplicons. (D) Western blot analysis showing USP11 knockout efficiency in hiPSCs cells. (E, F) The hiPSC-wildtype and hiPSC-USP11KO clones were characterized by (E) quantitative real-time reverse transcription-PCR using USP11 gene primers and (F) AP staining, Scale bar=200 μm. Data are presented as the mean and SD of three independent experiments (n=3). Two-way ANOVA followed by Tukey’s post hoc test was used to analyze. ***p<0.001.
Dnase (Dnase I, 30 U/ μ G Total Rna), supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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dnase i  (Promega)
90
Promega dnase i
a–c and e–g . LDH, IL-1β and IL-6 at 24h. a. Total EC RNA treated with RNAse I and RNAse III, RNAse III alone, or <t>DNAse</t> prior to stimulation of BMDC. b. BMM treated with viable or HKEC, or HKEC with 0.1µg/ml of different bacterial RNA; ribosomal (rRNA), messenger (mRNA), small (sRNA) or eukaryotic RNA (eukRNA). c. BMDC responses. Gro-RNA; in vitro transcribed EC Gro-operon RNA. d. Predicted secondary structure of Gro-RNA. Colour code indicates base pairing probability. e. BMM treated with in vitro transcribed Gro-RNA or Gro dsRNA alone or with HKEC. f. BMDC responses. Era-RNA and DNApol-RNA; in vitro transcribed EC Era-GTPase <t>and</t> <t>DNA-polymerase-III</t> RNA, respectively. g . BMM treated with different doses of unmodified (control), or modified Gro-RNA with HKEC (5’cap, 5’ m 7 G capping; CIP, calf intestinal phosphatase; 3’poly(A), 3’-polyadenylation). a–g. #, ‘not detected’, all RNA at 10µg/ml except as noted, data represent ≥5 experiments. h. Mice vaccinated and boosted twice with viable EC, HKEC or HKEC with 30µg total purified bacterial RNA (HKEC+RNA) (vaccination regimen in ). Class-specific anti- E. coli antibody serum titers at 25 days. *; p≤0.05, **; p≤0.01, ***; p≤0.001. All bars represent mean ± s.e.m.
Dnase I, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t7 endonuclease  (New England Biolabs)
86
New England Biolabs t7 endonuclease
a–c and e–g . LDH, IL-1β and IL-6 at 24h. a. Total EC RNA treated with RNAse I and RNAse III, RNAse III alone, or <t>DNAse</t> prior to stimulation of BMDC. b. BMM treated with viable or HKEC, or HKEC with 0.1µg/ml of different bacterial RNA; ribosomal (rRNA), messenger (mRNA), small (sRNA) or eukaryotic RNA (eukRNA). c. BMDC responses. Gro-RNA; in vitro transcribed EC Gro-operon RNA. d. Predicted secondary structure of Gro-RNA. Colour code indicates base pairing probability. e. BMM treated with in vitro transcribed Gro-RNA or Gro dsRNA alone or with HKEC. f. BMDC responses. Era-RNA and DNApol-RNA; in vitro transcribed EC Era-GTPase <t>and</t> <t>DNA-polymerase-III</t> RNA, respectively. g . BMM treated with different doses of unmodified (control), or modified Gro-RNA with HKEC (5’cap, 5’ m 7 G capping; CIP, calf intestinal phosphatase; 3’poly(A), 3’-polyadenylation). a–g. #, ‘not detected’, all RNA at 10µg/ml except as noted, data represent ≥5 experiments. h. Mice vaccinated and boosted twice with viable EC, HKEC or HKEC with 30µg total purified bacterial RNA (HKEC+RNA) (vaccination regimen in ). Class-specific anti- E. coli antibody serum titers at 25 days. *; p≤0.05, **; p≤0.01, ***; p≤0.001. All bars represent mean ± s.e.m.
T7 Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t7 endonuclease 1  (ToolGen Incorporated)
90
ToolGen Incorporated t7 endonuclease 1
a–c and e–g . LDH, IL-1β and IL-6 at 24h. a. Total EC RNA treated with RNAse I and RNAse III, RNAse III alone, or <t>DNAse</t> prior to stimulation of BMDC. b. BMM treated with viable or HKEC, or HKEC with 0.1µg/ml of different bacterial RNA; ribosomal (rRNA), messenger (mRNA), small (sRNA) or eukaryotic RNA (eukRNA). c. BMDC responses. Gro-RNA; in vitro transcribed EC Gro-operon RNA. d. Predicted secondary structure of Gro-RNA. Colour code indicates base pairing probability. e. BMM treated with in vitro transcribed Gro-RNA or Gro dsRNA alone or with HKEC. f. BMDC responses. Era-RNA and DNApol-RNA; in vitro transcribed EC Era-GTPase <t>and</t> <t>DNA-polymerase-III</t> RNA, respectively. g . BMM treated with different doses of unmodified (control), or modified Gro-RNA with HKEC (5’cap, 5’ m 7 G capping; CIP, calf intestinal phosphatase; 3’poly(A), 3’-polyadenylation). a–g. #, ‘not detected’, all RNA at 10µg/ml except as noted, data represent ≥5 experiments. h. Mice vaccinated and boosted twice with viable EC, HKEC or HKEC with 30µg total purified bacterial RNA (HKEC+RNA) (vaccination regimen in ). Class-specific anti- E. coli antibody serum titers at 25 days. *; p≤0.05, **; p≤0.01, ***; p≤0.001. All bars represent mean ± s.e.m.
T7 Endonuclease 1, supplied by ToolGen Incorporated, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dnase i amplification grade kit  (Thermo Fisher)
99
Thermo Fisher dnase i amplification grade kit
a–c and e–g . LDH, IL-1β and IL-6 at 24h. a. Total EC RNA treated with RNAse I and RNAse III, RNAse III alone, or <t>DNAse</t> prior to stimulation of BMDC. b. BMM treated with viable or HKEC, or HKEC with 0.1µg/ml of different bacterial RNA; ribosomal (rRNA), messenger (mRNA), small (sRNA) or eukaryotic RNA (eukRNA). c. BMDC responses. Gro-RNA; in vitro transcribed EC Gro-operon RNA. d. Predicted secondary structure of Gro-RNA. Colour code indicates base pairing probability. e. BMM treated with in vitro transcribed Gro-RNA or Gro dsRNA alone or with HKEC. f. BMDC responses. Era-RNA and DNApol-RNA; in vitro transcribed EC Era-GTPase <t>and</t> <t>DNA-polymerase-III</t> RNA, respectively. g . BMM treated with different doses of unmodified (control), or modified Gro-RNA with HKEC (5’cap, 5’ m 7 G capping; CIP, calf intestinal phosphatase; 3’poly(A), 3’-polyadenylation). a–g. #, ‘not detected’, all RNA at 10µg/ml except as noted, data represent ≥5 experiments. h. Mice vaccinated and boosted twice with viable EC, HKEC or HKEC with 30µg total purified bacterial RNA (HKEC+RNA) (vaccination regimen in ). Class-specific anti- E. coli antibody serum titers at 25 days. *; p≤0.05, **; p≤0.01, ***; p≤0.001. All bars represent mean ± s.e.m.
Dnase I Amplification Grade Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dnase i amplification grade kit - by Bioz Stars, 2026-02
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m0302l  (New England Biolabs)
96
New England Biolabs m0302l
a–c and e–g . LDH, IL-1β and IL-6 at 24h. a. Total EC RNA treated with RNAse I and RNAse III, RNAse III alone, or <t>DNAse</t> prior to stimulation of BMDC. b. BMM treated with viable or HKEC, or HKEC with 0.1µg/ml of different bacterial RNA; ribosomal (rRNA), messenger (mRNA), small (sRNA) or eukaryotic RNA (eukRNA). c. BMDC responses. Gro-RNA; in vitro transcribed EC Gro-operon RNA. d. Predicted secondary structure of Gro-RNA. Colour code indicates base pairing probability. e. BMM treated with in vitro transcribed Gro-RNA or Gro dsRNA alone or with HKEC. f. BMDC responses. Era-RNA and DNApol-RNA; in vitro transcribed EC Era-GTPase <t>and</t> <t>DNA-polymerase-III</t> RNA, respectively. g . BMM treated with different doses of unmodified (control), or modified Gro-RNA with HKEC (5’cap, 5’ m 7 G capping; CIP, calf intestinal phosphatase; 3’poly(A), 3’-polyadenylation). a–g. #, ‘not detected’, all RNA at 10µg/ml except as noted, data represent ≥5 experiments. h. Mice vaccinated and boosted twice with viable EC, HKEC or HKEC with 30µg total purified bacterial RNA (HKEC+RNA) (vaccination regimen in ). Class-specific anti- E. coli antibody serum titers at 25 days. *; p≤0.05, **; p≤0.01, ***; p≤0.001. All bars represent mean ± s.e.m.
M0302l, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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t7 endonuclease i t7ei  (Danaher Inc)
86
Danaher Inc t7 endonuclease i t7ei
a–c and e–g . LDH, IL-1β and IL-6 at 24h. a. Total EC RNA treated with RNAse I and RNAse III, RNAse III alone, or <t>DNAse</t> prior to stimulation of BMDC. b. BMM treated with viable or HKEC, or HKEC with 0.1µg/ml of different bacterial RNA; ribosomal (rRNA), messenger (mRNA), small (sRNA) or eukaryotic RNA (eukRNA). c. BMDC responses. Gro-RNA; in vitro transcribed EC Gro-operon RNA. d. Predicted secondary structure of Gro-RNA. Colour code indicates base pairing probability. e. BMM treated with in vitro transcribed Gro-RNA or Gro dsRNA alone or with HKEC. f. BMDC responses. Era-RNA and DNApol-RNA; in vitro transcribed EC Era-GTPase <t>and</t> <t>DNA-polymerase-III</t> RNA, respectively. g . BMM treated with different doses of unmodified (control), or modified Gro-RNA with HKEC (5’cap, 5’ m 7 G capping; CIP, calf intestinal phosphatase; 3’poly(A), 3’-polyadenylation). a–g. #, ‘not detected’, all RNA at 10µg/ml except as noted, data represent ≥5 experiments. h. Mice vaccinated and boosted twice with viable EC, HKEC or HKEC with 30µg total purified bacterial RNA (HKEC+RNA) (vaccination regimen in ). Class-specific anti- E. coli antibody serum titers at 25 days. *; p≤0.05, **; p≤0.01, ***; p≤0.001. All bars represent mean ± s.e.m.
T7 Endonuclease I T7ei, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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t7 endonuclease  (Fisher Scientific)
90
Fisher Scientific t7 endonuclease
a–c and e–g . LDH, IL-1β and IL-6 at 24h. a. Total EC RNA treated with RNAse I and RNAse III, RNAse III alone, or <t>DNAse</t> prior to stimulation of BMDC. b. BMM treated with viable or HKEC, or HKEC with 0.1µg/ml of different bacterial RNA; ribosomal (rRNA), messenger (mRNA), small (sRNA) or eukaryotic RNA (eukRNA). c. BMDC responses. Gro-RNA; in vitro transcribed EC Gro-operon RNA. d. Predicted secondary structure of Gro-RNA. Colour code indicates base pairing probability. e. BMM treated with in vitro transcribed Gro-RNA or Gro dsRNA alone or with HKEC. f. BMDC responses. Era-RNA and DNApol-RNA; in vitro transcribed EC Era-GTPase <t>and</t> <t>DNA-polymerase-III</t> RNA, respectively. g . BMM treated with different doses of unmodified (control), or modified Gro-RNA with HKEC (5’cap, 5’ m 7 G capping; CIP, calf intestinal phosphatase; 3’poly(A), 3’-polyadenylation). a–g. #, ‘not detected’, all RNA at 10µg/ml except as noted, data represent ≥5 experiments. h. Mice vaccinated and boosted twice with viable EC, HKEC or HKEC with 30µg total purified bacterial RNA (HKEC+RNA) (vaccination regimen in ). Class-specific anti- E. coli antibody serum titers at 25 days. *; p≤0.05, **; p≤0.01, ***; p≤0.001. All bars represent mean ± s.e.m.
T7 Endonuclease, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/t7 endonuclease/product/Fisher Scientific
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porcine pancreatic dnase  (Millipore)
90
Millipore porcine pancreatic dnase
a–c and e–g . LDH, IL-1β and IL-6 at 24h. a. Total EC RNA treated with RNAse I and RNAse III, RNAse III alone, or <t>DNAse</t> prior to stimulation of BMDC. b. BMM treated with viable or HKEC, or HKEC with 0.1µg/ml of different bacterial RNA; ribosomal (rRNA), messenger (mRNA), small (sRNA) or eukaryotic RNA (eukRNA). c. BMDC responses. Gro-RNA; in vitro transcribed EC Gro-operon RNA. d. Predicted secondary structure of Gro-RNA. Colour code indicates base pairing probability. e. BMM treated with in vitro transcribed Gro-RNA or Gro dsRNA alone or with HKEC. f. BMDC responses. Era-RNA and DNApol-RNA; in vitro transcribed EC Era-GTPase <t>and</t> <t>DNA-polymerase-III</t> RNA, respectively. g . BMM treated with different doses of unmodified (control), or modified Gro-RNA with HKEC (5’cap, 5’ m 7 G capping; CIP, calf intestinal phosphatase; 3’poly(A), 3’-polyadenylation). a–g. #, ‘not detected’, all RNA at 10µg/ml except as noted, data represent ≥5 experiments. h. Mice vaccinated and boosted twice with viable EC, HKEC or HKEC with 30µg total purified bacterial RNA (HKEC+RNA) (vaccination regimen in ). Class-specific anti- E. coli antibody serum titers at 25 days. *; p≤0.05, **; p≤0.01, ***; p≤0.001. All bars represent mean ± s.e.m.
Porcine Pancreatic Dnase, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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porcine pancreatic dnase - by Bioz Stars, 2026-02
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dnase  (Millipore)
90
Millipore dnase
a–c and e–g . LDH, IL-1β and IL-6 at 24h. a. Total EC RNA treated with RNAse I and RNAse III, RNAse III alone, or <t>DNAse</t> prior to stimulation of BMDC. b. BMM treated with viable or HKEC, or HKEC with 0.1µg/ml of different bacterial RNA; ribosomal (rRNA), messenger (mRNA), small (sRNA) or eukaryotic RNA (eukRNA). c. BMDC responses. Gro-RNA; in vitro transcribed EC Gro-operon RNA. d. Predicted secondary structure of Gro-RNA. Colour code indicates base pairing probability. e. BMM treated with in vitro transcribed Gro-RNA or Gro dsRNA alone or with HKEC. f. BMDC responses. Era-RNA and DNApol-RNA; in vitro transcribed EC Era-GTPase <t>and</t> <t>DNA-polymerase-III</t> RNA, respectively. g . BMM treated with different doses of unmodified (control), or modified Gro-RNA with HKEC (5’cap, 5’ m 7 G capping; CIP, calf intestinal phosphatase; 3’poly(A), 3’-polyadenylation). a–g. #, ‘not detected’, all RNA at 10µg/ml except as noted, data represent ≥5 experiments. h. Mice vaccinated and boosted twice with viable EC, HKEC or HKEC with 30µg total purified bacterial RNA (HKEC+RNA) (vaccination regimen in ). Class-specific anti- E. coli antibody serum titers at 25 days. *; p≤0.05, **; p≤0.01, ***; p≤0.001. All bars represent mean ± s.e.m.
Dnase, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Image Search Results


Generation of USP11 gene disruption in human induced pluripotent stem cells (hiPSCs). (A) The location of designed sgRNAs targeting USP11 gene. (B) The efficiency of sgRNAs targeting USP11 was validated by western blot using the USP11 antibody. (C) The USP11 knockout clone in hiPSCs cell line was screened by T7 endonuclease 1 (T7E1) assay. The T7E1 positive clones showing cleavage are represented in red color. The arrowhead indicates the cleaved bands of the polymerase chain reaction (PCR) amplicons. (D) Western blot analysis showing USP11 knockout efficiency in hiPSCs cells. (E, F) The hiPSC-wildtype and hiPSC-USP11KO clones were characterized by (E) quantitative real-time reverse transcription-PCR using USP11 gene primers and (F) AP staining, Scale bar=200 μm. Data are presented as the mean and SD of three independent experiments (n=3). Two-way ANOVA followed by Tukey’s post hoc test was used to analyze. ***p<0.001.

Journal: International Journal of Stem Cells

Article Title: Loss of Ubiquitin-Specific Protease 11 Mitigates Pulmonary Fibrosis in Human Pluripotent Stem Cell-Derived Alveolar Organoids

doi: 10.15283/ijsc25011

Figure Lengend Snippet: Generation of USP11 gene disruption in human induced pluripotent stem cells (hiPSCs). (A) The location of designed sgRNAs targeting USP11 gene. (B) The efficiency of sgRNAs targeting USP11 was validated by western blot using the USP11 antibody. (C) The USP11 knockout clone in hiPSCs cell line was screened by T7 endonuclease 1 (T7E1) assay. The T7E1 positive clones showing cleavage are represented in red color. The arrowhead indicates the cleaved bands of the polymerase chain reaction (PCR) amplicons. (D) Western blot analysis showing USP11 knockout efficiency in hiPSCs cells. (E, F) The hiPSC-wildtype and hiPSC-USP11KO clones were characterized by (E) quantitative real-time reverse transcription-PCR using USP11 gene primers and (F) AP staining, Scale bar=200 μm. Data are presented as the mean and SD of three independent experiments (n=3). Two-way ANOVA followed by Tukey’s post hoc test was used to analyze. ***p<0.001.

Article Snippet: The heteroduplex DNA was then treated with 5 units of T7 endonuclease 1 (T7E1; New England Biolabs) for 15 to 20 minutes at 37℃, followed by 2% agarose gel electrophoresis.

Techniques: Disruption, Western Blot, Knock-Out, Clone Assay, Polymerase Chain Reaction, Reverse Transcription, Staining

a–c and e–g . LDH, IL-1β and IL-6 at 24h. a. Total EC RNA treated with RNAse I and RNAse III, RNAse III alone, or DNAse prior to stimulation of BMDC. b. BMM treated with viable or HKEC, or HKEC with 0.1µg/ml of different bacterial RNA; ribosomal (rRNA), messenger (mRNA), small (sRNA) or eukaryotic RNA (eukRNA). c. BMDC responses. Gro-RNA; in vitro transcribed EC Gro-operon RNA. d. Predicted secondary structure of Gro-RNA. Colour code indicates base pairing probability. e. BMM treated with in vitro transcribed Gro-RNA or Gro dsRNA alone or with HKEC. f. BMDC responses. Era-RNA and DNApol-RNA; in vitro transcribed EC Era-GTPase and DNA-polymerase-III RNA, respectively. g . BMM treated with different doses of unmodified (control), or modified Gro-RNA with HKEC (5’cap, 5’ m 7 G capping; CIP, calf intestinal phosphatase; 3’poly(A), 3’-polyadenylation). a–g. #, ‘not detected’, all RNA at 10µg/ml except as noted, data represent ≥5 experiments. h. Mice vaccinated and boosted twice with viable EC, HKEC or HKEC with 30µg total purified bacterial RNA (HKEC+RNA) (vaccination regimen in ). Class-specific anti- E. coli antibody serum titers at 25 days. *; p≤0.05, **; p≤0.01, ***; p≤0.001. All bars represent mean ± s.e.m.

Journal: Nature

Article Title: Sensing prokaryotic mRNA signifies microbial viability and promotes immunity

doi: 10.1038/nature10072

Figure Lengend Snippet: a–c and e–g . LDH, IL-1β and IL-6 at 24h. a. Total EC RNA treated with RNAse I and RNAse III, RNAse III alone, or DNAse prior to stimulation of BMDC. b. BMM treated with viable or HKEC, or HKEC with 0.1µg/ml of different bacterial RNA; ribosomal (rRNA), messenger (mRNA), small (sRNA) or eukaryotic RNA (eukRNA). c. BMDC responses. Gro-RNA; in vitro transcribed EC Gro-operon RNA. d. Predicted secondary structure of Gro-RNA. Colour code indicates base pairing probability. e. BMM treated with in vitro transcribed Gro-RNA or Gro dsRNA alone or with HKEC. f. BMDC responses. Era-RNA and DNApol-RNA; in vitro transcribed EC Era-GTPase and DNA-polymerase-III RNA, respectively. g . BMM treated with different doses of unmodified (control), or modified Gro-RNA with HKEC (5’cap, 5’ m 7 G capping; CIP, calf intestinal phosphatase; 3’poly(A), 3’-polyadenylation). a–g. #, ‘not detected’, all RNA at 10µg/ml except as noted, data represent ≥5 experiments. h. Mice vaccinated and boosted twice with viable EC, HKEC or HKEC with 30µg total purified bacterial RNA (HKEC+RNA) (vaccination regimen in ). Class-specific anti- E. coli antibody serum titers at 25 days. *; p≤0.05, **; p≤0.01, ***; p≤0.001. All bars represent mean ± s.e.m.

Article Snippet: Contaminating genomic DNA was removed by DNase digestion (DNase I, Promega).

Techniques: In Vitro, Control, Modification, Purification